To investigate the effect of conjunction position on fatigue performance of laminated smart structures， a study of macro fiber composite（MFC） and glass fiber reinforced polymer （GFRP） laminated smart structure is presented. Experiments on two types of specimens with different conjunction positions are carried out， and the results indicate that the fatigue life of smart structure is higher when MFC is in the middle layer of structure than at the outermost layer. A finite element model （FEM） model is established and validated by the error within 7% comparing with the load-strain data during experiment. Fatigue life prediction using a linear S-N curve shows good agreement with experimental results. The effect of the MFC conjunction position on the fatigue life of the smart structure under different load is further analyzed. Under uniaxial load， the interfacial stress decreases and the fatigue life increases as the MFC conjunction position shifts from the outermost layer to the middle layer， and this improvement is dominated by a significant reduction in tensile stress. Besides， stress concentration occurs near the short edges of the rectangular MFC under uniaxial tensile load， and around the perimeter of the MFC under uniaxial torsional load. Under multiaxial load， compared to the outermost-layer conjunction， the middle-layer conjunction almost eliminates interlaminar tensile stress and reduces the effective shear stress to varying degrees， leading to enhanced fatigue life. The degree of fatigue life improvement is significantly affected by the load ratio， and the optimal improvement occurs at a load ratio of 0.273 4.