When high-resolution satellite is imaging on orbit, the relative motion between the sensitive optical elements inside the camera may be caused by micro-vibration, which results in degraded images. In order to study the effect of micro-vibration on high resolution satellite imaging, a micro-vibration impact analysis method of space camera based on target image is proposed. Firstly, the different factors of micro-vibration which affect the image quality in different degrees are introduced. Then, the method which can realize the direct measurement of image motion in the image is proposed. The method can analyze the impact of micro-vibration on the space camera directly and get the accurate number of the image motion, which can provide the basis for the influence analysis of the satellite image quality and the final correction. Finally, we take a factual space camera for example to calculate and analyze the test data by using the model on the condition of the satellite. We get the image motion caused by the micro-vibration on focal plane and accomplish the influence analysis method of micro-vibration on high resolution satellite imaging. It is concluded that the proposed method is reasonable and practical．The method proposed by the paper can be reference for improved design and vibration suppression.