SIMU’s accelerometer is usually calibrated by turntable and the accelerometer calibration accuracy is directly affected by the turntable’s unbalanced error. In order to quantify the unbalanced error influences, based on the static twelve-position model, the relation between unbalanced error and calibration is derived. The simulation analysis results indicate that the fixed error is significantly influenced by turntable’s unbalanced error, while the bias and scale factor error are little influenced by it. The results can provide a reference on the out-of-tolerance calibration parameters during tests.