Spaceflight AIT center is one of the important environmental sources of spacecrafts carrying microorganisms. Therefore, the research of microorganism identification and its disinfection methods are of great importance for developing techniques to prevent and control of mould contamination of spacecrafts. This study reports the typical fungal colonies and spores morphology of eight species isolated from the spacecraft AIT center, including Penicillium sp, Aspergillus sp, Cladosporium sp etc, which provids reference for mold identification and the construction of strain bank. The disinfection effects of disinfectants, UVC irradiation and heat treatment on those moulds are then studied, so as to provide scientific evidence for prevention and control of mold technology for spacecrafts. The results show that the sensitivity of fungi with different species to the three disinfection methods has great differences. Combined with the diversity of AIT central fungi species in different regions of China, we should select different disinfection methods scientifically or apply multiple methods when carrying out the disinfection work at AIT central.