Seaplane anti-waves ability evaluation is an important work for seaplane fly test. The analytic hierarchy process is used to evaluate the seaplane anti-waves ability. The method fully considers the complicacy of anti-waves ability evaluation, makes full use of expert engineering experience, distinguishes the weight of different evaluation parameters, and evaluates the seaplane anti-waves ability. By the HO 300 light amphibious aircraft example, the evaluation result shows that its safe taking-off and landing probability reaches to 88.5% in 0.3 m wave height. Experimental results validate the feasibility and validity of the method.