In order to study the effect of the vacuum insulation panel (VIP) on the uniformity of temperature distribution inside reefer container， a physical and mathematical model of the air flow and heat transfer inside a 20-feet reefer container is established. The standard k-ε turbulent model is adopted to simulate the uniformity of temperature distribution inside reefer container equipped with VIP or polyurethane under the steady state. The result shows that the uniformity of temperature distribution inside reefer container equipped with VIP is better than that with the polyurethane. Meanwhile， the average temperature is reduced by 0.8 K， and the simulation result has significance to improve the quality of refrigerated transport. At last， an experiment on a 20-feet polyurethane reefer container is conducted， and the experimental result is well agreeable with the simulation， thus verifying the accuracy of the mathematical mode.