RuxSey/graphene catalysts are synthesized by the one-pot solvothermal method, with the RuCl3·xH2O and SeO2 as precursors and the reduced graphene oxide as carrier. The influence of ethanol and ethylene glycol on the morphology performance of the catalysts is investigated. By utilizing transmission electron microscopy (TEM), X-ray diffraction (XRD), Raman spectroscopy (Raman) and rotating disk electrode (RDE) techniques, the physical property and activity for oxygen reduction reaction (ORR) of as-prepared electrocatalysts are characterized, respectively. The results suggest that RuxSey particles can be evenly dispersed on graphene layers and RuxSey/graphene catalysts prepared in ethylene glycol are well crystalized. The presence of ethylene glycol can contribute to the reduction process of graphene oxide and the increase in its specific surface area, thus leading to a relatively high catalytic activity of the catalyst upon ORR in 0.1 mol/L KOH.