With the increase of cognition and deep exploration for outer space, the contamination problem between stars has aroused more and more attention. Planetary prevention has become an important topic in space exploration field. An experimental study on microbial contamination in enclosed cabinof Lunar Palace 1 (LP1) ground-basedbioregenerative life support system (BLSS) is conducted. The development and succession law of dominant microorganisms obtained through experiments can give a base for the construction of more targeted microorganisms preventive and control measures in enclosed BLSS experiments in the future, provide important experience for solving the microbial contamination problem in a closed space.